The kit says 68 °C. The textbook says 72.
Which one is your bench?

Aliquot reads manufacturer manuals, published protocols and biosafety guidance through a Sanity Knowledge Base, keeps every value as a sourced claim in a structured ledger, and applies them in order: your lab’s SOP, then the manual for the product in your hand, then everything else. The numbers — PCR programs, master mixes, recipes, dilutions — are computed from the data, not remembered by a model.